Design of 28 GHz 4x4 RF Beamforming Array for 5G Radio Front-Ends

Current state of wireless infrastructure sees mass migration to higher frequencies as much of the already used spectrum is insufficient in supporting the influx of numerous users and various data intensive mobile applications. Data rates are projected to increase by an order of magnitude and harnessing the necessary bandwidth below 6 GHz is not feasible. A move to higher frequencies sees not only increased fractional bandwidth, but also significantly enhanced antenna apertures as a result of beamforming capabilities. Due to device level complications with frequencies nearing the unit gain frequency of transistor technology, high output power is seldom found, and in conjunction with severe path loss, communication links cannot be established without the usage of antenna arrays. Phased array systems offer significant upside to the traditional array implementation as it permits reconfigurable directive communication. However, Ka-Band phased arrays still struggle to arrive at a reasonable tradeoff between design complexity, cost and performance. With a divide between both organic and printed circuit board (PCB) based approaches to the development of an antenna-in-package (AiP), this thesis sides with the latter. An antenna-on-PCB variant of the AiP is developed, which implements both commercially available RF laminates and RFIC front end modules to produce a 28 GHz 4x4 RF beamforming phased array that is found to exhibit extremely low loss (-0.66 dB), adequate scan volume (+/- 45 degrees, in E and H planes) and large bandwidth (3 GHz) for a single layer, non-isolated patch antenna design. Unit cell, infinite array analysis is emphasized and lattice resizing is leveraged to obtain desired scan performance, while significantly reducing design complexity via the absence of intricate isolation enhancement techniques. In an effort to aid in application based design, the AiP is extended to application of linearization where it is found that the inclusion of dummy elements along the perimeter of the package not only serve as element pattern enhancement, but also provide reliable means of output signal capture. Negating the traditional transmitter observation receiver (TOR) architecture, the AiP design as a TOR for millimeter-wave communication proves optimistic in the quest for maximum system efficiency

A new biaxial apparatus integrated within a pressure vessel to test the physical properties of brittle rock: the state-of-the-art

A main goal of the European Research Council, Starting Grant, GLASS (InteGrated Laboratories to investigate the mechanics of ASeismic vs Seismic faulting), is to develop a prototype rock deformation biaxial apparatus to examine the physical properties of brittle rocks. Two layers of fault rock are sandwiched between three steel block by a normal load applied using a horizontal oil-dynamics piston. A vertical oil-dynamics piston pushes the internal rock sample of the sandwich in order to slide at constant velocity. With GLASS we are going to build-up a confining pressure around the rock samples under load stress (tri-axial mode) and to measure the fluid flow properties of the rock during the deformation. Working in tri-axial mode with a fluid circulation, the machine is able to measure and to characterize frictional properties of faults on the sample for a wide spectrum of realistic conditions. We have concurrently been working to improve the control and the acquisition system for having a machine very flexible and easy to use for several applications and capable to detect different signals on the rock during frictional sliding in a fluid-rich environment with the goal of comparing these signals to those observed in nature. We began designing the servo controlled machine in October 2010 and have recently installed the apparatus in the HP-HT lab at the INGV in Rome. First tests of this biaxial apparatus confirm the main target of the project

Clinical-Genetic Features Influencing Disability in Spastic Paraplegia Type 4: A Cross-sectional Study by the Italian DAISY Network

Background and objectives: Hereditary spastic paraplegias (HSPs) are a group of inherited rare neurologic disorders characterized by length-dependent degeneration of the corticospinal tracts and dorsal columns, whose prominent clinical feature is represented by spastic gait. Spastic paraplegia type 4 (SPG4, SPAST-HSP) is the most common form. We present both clinical and molecular findings of a large cohort of patients, with the aim of (1) defining the clinical spectrum of SPAST-HSP in Italy; (2) describing their molecular features; and (3) assessing genotype-phenotype correlations to identify features associated with worse disability. Methods: A cross-sectional retrospective study with molecular and clinical data collected in an anonymized database was performed. Results: A total of 723 Italian patients with SPAST-HSP (58% men) from 316 families, with a median age at onset of 35 years, were included. Penetrance was 97.8%, with men showing higher Spastic Paraplegia Rating Scale (SPRS) scores (19.67 ± 12.58 vs 16.15 ± 12.61, p = 0.009). In 26.6% of patients with SPAST-HSP, we observed a complicated phenotype, mainly including intellectual disability (8%), polyneuropathy (6.7%), and cognitive decline (6.5%). Late-onset cases seemed to progress more rapidly, and patients with a longer disease course displayed a more severe neurologic disability, with higher SPATAX (3.61 ± 1.46 vs 2.71 ± 1.20, p < 0.001) and SPRS scores (22.63 ± 11.81 vs 12.40 ± 8.83, p < 0.001). Overall, 186 different variants in the SPAST gene were recorded, of which 48 were novel. Patients with SPAST-HSP harboring missense variants displayed intellectual disability (14.5% vs 4.4%, p < 0.001) more frequently, whereas patients with truncating variants presented more commonly cognitive decline (9.7% vs 2.6%, p = 0.001), cerebral atrophy (11.2% vs 3.4%, p = 0.003), lower limb spasticity (61.5% vs 44.5%), urinary symptoms (50.0% vs 31.3%, p < 0.001), and sensorimotor polyneuropathy (11.1% vs 1.1%, p < 0.001). Increasing disease duration (DD) and abnormal motor evoked potentials (MEPs) were also associated with increased likelihood of worse disability (SPATAX score>3). Discussion: The SPAST-HSP phenotypic spectrum in Italian patients confirms a predominantly pure form of HSP with mild-to-moderate disability in 75% of cases, and slight prevalence of men, who appeared more severely affected. Early-onset cases with intellectual disability were more frequent among patients carrying missense SPAST variants, whereas patients with truncating variants showed a more complicated disease. Both longer DD and altered MEPs are associated with worse disability

A 5-year clinical follow-up study from the Italian National Registry for FSHD.

BACKGROUND: The natural history of facioscapulohumeral muscular dystrophy (FSHD) is undefined. METHODS: An observational cohort study was conducted in 246 FSHD1 patients. We split the analysis between index cases and carrier relatives and we classified all patients using the Comprehensive Clinical Evaluation Form (CCEF). The disease progression was measured as a variation of the FSHD score performed at baseline and at the end of 5-year follow-up (ΔFSHD score). FINDINGS: Disease worsened in 79.4% (112/141) of index cases versus 38.1% (40/105) of carrier relatives and advanced more rapidly in index cases (ΔFSHD score 2.3 versus 1.2). The 79.1% (38/48) of asymptomatic carriers remained asymptomatic. The highest ΔFSHD score (1.7) was found in subject with facial and scapular weakness at baseline (category A), whereas in subjects with incomplete phenotype (facial or scapular weakness, category B) had lower ΔFSHD score (0.6) p < 0.0001. CONCLUSIONS: The progression of disease is different between index cases and carrier relatives and the assessment of the CCEF categories has strong prognostic effect in FSHD1 patients

Large genotype-phenotype study in carriers of D4Z4 borderline alleles provides guidance for facioscapulohumeral muscular dystrophy diagnosis.

Facioscapulohumeral muscular dystrophy (FSHD) is a myopathy with prevalence of 1 in 20,000. Almost all patients affected by FSHD carry deletions of an integral number of tandem 3.3 kilobase repeats, termed D4Z4, located on chromosome 4q35. Assessment of size of D4Z4 alleles is commonly used for FSHD diagnosis. However, the extended molecular testing has expanded the spectrum of clinical phenotypes. In particular, D4Z4 alleles with 9-10 repeat have been found in healthy individuals, in subjects with FSHD or affected by other myopathies. These findings weakened the strict relationship between observed phenotypes and their underlying genotypes, complicating the interpretation of molecular findings for diagnosis and genetic counseling. In light of the wide clinical variability detected in carriers of D4Z4 alleles with 9-10 repeats, we applied a standardized methodology, the Comprehensive Clinical Evaluation Form (CCEF), to describe and characterize the phenotype of 244 individuals carrying D4Z4 alleles with 9-10 repeats (134 index cases and 110 relatives). The study shows that 54.5% of index cases display a classical FSHD phenotype with typical facial and scapular muscle weakness, whereas 20.1% present incomplete phenotype with facial weakness or scapular girdle weakness, 6.7% display minor signs such as winged scapula or hyperCKemia, without functional motor impairment, and 18.7% of index cases show more complex phenotypes with atypical clinical features. Family studies revealed that 70.9% of relatives carrying 9-10 D4Z4 reduced alleles has no motor impairment, whereas a few relatives (10.0%) display a classical FSHD phenotype. Importantly all relatives of index cases with no FSHD phenotype were healthy carriers. These data establish the low penetrance of D4Z4 alleles with 9-10 repeats. We recommend the use of CCEF for the standardized clinical assessment integrated by family studies and further molecular investigation for appropriate diagnosis and genetic counseling. Especially in presence of atypical phenotypes and/or sporadic cases with all healthy relatives is not possible to perform conclusive diagnosis of FSHD, but all these cases need further studies for a proper diagnosis, to search novel causative genetic defects or investigate environmental factors or co-morbidities that may trigger the pathogenic process. These evidences are also fundamental for the stratification of patients eligible for clinical trials. Our work reinforces the value of large genotype-phenotype studies to define criteria for clinical practice and genetic counseling in rare diseases

Feeling the Heat: The Campylobacter jejuni HrcA Transcriptional Repressor Is an Intrinsic Protein Thermosensor

The heat-shock response, a universal protective mechanism consisting of a transcriptional reprogramming of the cellular transcriptome, results in the accumulation of proteins which counteract the deleterious effects of heat-stress on cellular polypeptides. To quickly respond to thermal stress and trigger the heat-shock response, bacteria rely on different mechanisms to detect temperature variations, which can involve nearly all classes of biological molecules. In Campylobacter jejuni the response to heat-shock is transcriptionally controlled by a regulatory circuit involving two repressors, HspR and HrcA. In the present work we show that the heat-shock repressor HrcA acts as an intrinsic protein thermometer. We report that a temperature upshift up to 42 °C negatively affects HrcA DNA-binding activity to a target promoter, a condition required for de-repression of regulated genes. Furthermore, we show that this impairment of HrcA binding at 42 °C is irreversible in vitro, as DNA-binding was still not restored by reversing the incubation temperature to 37 °C. On the other hand, we demonstrate that the DNA-binding activity of HspR, which controls, in combination with HrcA, the transcription of chaperones’ genes, is unaffected by heat-stress up to 45 °C, portraying this master repressor as a rather stable protein. Additionally, we show that HrcA binding activity is enhanced by the chaperonin GroE, upon direct protein–protein interaction. In conclusion, the results presented in this work establish HrcA as a novel example of intrinsic heat-sensing transcriptional regulator, whose DNA-binding activity is positively modulated by the GroE chaperonin

A new biaxial apparatus integrated within a pressure vessel to test the physical properties of brittle rock: the state-of-the-art

A main goal of the European Research Council, Starting Grant, GLASS (InteGrated Laboratories to investigate the mechanics of ASeismic vs Seismic faulting), is to develop a prototype rock deformation biaxial apparatus to examine the physical properties of brittle rocks. Two layers of fault rock are sandwiched between three steel block by a normal load applied using a horizontal oil-dynamics piston. A vertical oil-dynamics piston pushes the internal rock sample of the sandwich in order to slide at constant velocity. With GLASS we are going to build-up a confining pressure around the rock samples under load stress (tri-axial mode) and to measure the fluid flow properties of the rock during the deformation. Working in tri-axial mode with a fluid circulation, the machine is able to measure and to characterize frictional properties of faults on the sample for a wide spectrum of realistic conditions. We have concurrently been working to improve the control and the acquisition system for having a machine very flexible and easy to use for several applications and capable to detect different signals on the rock during frictional sliding in a fluid-rich environment with the goal of comparing these signals to those observed in nature. We began designing the servo controlled machine in October 2010 and have recently installed the apparatus in the HP-HT lab at the INGV in Rome. First tests of this biaxial apparatus confirm the main target of the project.UnpublishedHarvard University, Boston2.3. TTC - Laboratori di chimica e fisica delle rocceope

Schorl breakdown at upper mantle conditions. Insights from an experimental study at 3.5 GPa

Hydrogen and B input throughout the Earth’s mantle is continuously fed through a sequence of dehydration and breakdown reactions of hydrous and B-bearing mineral phases stable at different conditions along the subducting slabs. Therefore, the stability of minerals hosting these elements plays a fundamental role. Tourmaline hosts very large amounts of B (up to 14 wt% of B2O3) along with hydroxyl groups (up to 4 wt% of H2O), thus representing a crucial mineral to investigate the fate of B and H in diverse geological settings. The recent finding of tourmaline minerals in ultra-high pressure metamorphic rocks has raised important questions about the actual tourmaline stability field, paying special attention to the high pressure and temperature stability limits of the various tourmaline species. A single-phase system made of natural schorl with the highest Fe2+ concentration known so far (about 18 wt% of FeO) was studied at a fixed pressure (3.5 GPa) and several temperatures (500, 700, 750, 800, 850 and 950 ◦C) to preliminarily constrain its stability conditions, breakdown mechanisms and breakdown products. Experiments at high pressure-high temperature conditions were performed using a multi anvil apparatus under buffered oxygen fugacity through a Re/ReO2 solid mixture. The experimental products were characterized through a multi-analytical approach consisting in Scanning Electron Microscopy imaging and Energy Dispersive System spectra acquisition, Electron MicroProbe analysis, powder X-Ray Diffraction, 57Fe M ̈ossbauer spectroscopy and reflectance Fourier Transform infrared spectroscopy. At 3.5 GPa and T ranging from 500 up to 700 ◦C, the schorl experienced a partial Fe oxidation coupled with dehydrogenation: Fe2+ + (OH) →Fe3+ + O2 + 0.5H2 (g) The observed Fe oxidation was limited to 30% (significantly lower than the full oxidation observed in ex- periments performed in air at room pressure), suggesting that oxidation-dehydrogenation is indeed a thermally activated process, but both environmental pressure and oxygen fugacity are important governing factors. In the pure schorl system at 3.5 GPa, the structural breakdown started at T = 700 ◦C and ended at 850 ◦C, resulting in the formation of almandine garnet as the first breakdown product together with topaz and a B-rich liquid phase: Na Fe2+ 2 Al) Al5 Fe2+)(Si6O18)(BO3 )3(OH)3 (OH, F) schorl → →Fe2+ 3 Al2 (SiO4 )3 almandine + Fe3+, Al)2SiO4(OH, F)2 topaz + 2SiO2 + Al2O3 + 0.5Na2 O + 1.5B2O3 + H2 O melt At 3.5 GPa and T ≥ 850 ◦ C, tourmaline, garnet and topaz were not observed anymore and kyanite, prismatine- and boromullite-like phases and corundum became stable. Both prismatine-like and boromullite-like phases identified by stoichiometry can incorporate B from the B-rich hydrous melt formed after schorl breakdown and may carry it to lower depths. From our work it follows that the schorl-bearing granitoid rocks (or sediments) have the potential to form hydrous B-bearing metasomatic melts at 3.5 GPa and T ≥ 700 ◦C. In cold subduction environments, between the 700–800 ◦C isotherms, the schorl is expected to be stable up to ~100 km depth along the subducting slab, although an excess SiO2 might be responsible for a reduction in tourmaline stability. The role of tourmaline companion minerals on its breakdown conditions and products is left as future issue when a multi-phase system will be considered